Джош Кітон

Peter Parker / Spider Man (voice)

Лейсі Шебер

Gwen Stacy (voice)

Joshua LeBar

Flash Thompson (voice), Flash Thompson / Car Thief #1 (voice)

Ґрей Ґріффін

Stephanie Briggs (voice), Sally Avril / Computer Voice (voice), Betty Brant (voice), Sally Avril / Erin (voice), Sally Avril / Voice of OsCorp (voice), Sally Avril / Coffee Clerk (voice), Sally Avril / Betty Brant (voice), Voice of OsCorp (voice), Sally Avril / Bette Brant (voice), Sally Avril (voice)

Аланна Убах

Liz Allan (voice), Liz Allan / Helena (voice), Liz Allan / Trina (voice)

Vanessa Marshall

Mary Jane Watson / Hermia (voice), Mary Jane Watson (voice), Mary Jane Watson / Rosie Thompson (voice)

Джеймс Арнольд Тейлор

Patch / Homunculi (voice), Harry Osborn / Alan O'Neil (voice), Harry Osborn / Frederick Foswell (voice), Harry Osborn (voice), Harry Osborn / Patch / Frederick Foswell (voice)

Deborah Strang

May Parker (voice)

Клансі Браун

George Stacy (voice), Rhino / George Stacy (voice), Alex O'Hirn (voice), Alex O'Hirn / Rhino / George Stacy (voice), Alex O'Hirn / Ox (voice)

Alan Rachins

Norman Osborn (voice)

Філ Ламарр

Rand Robertson / Robbie Robertson (voice), Rand Robertson (voice), Rand Robertson / Fancy Dan (voice), Robbie Robertson (voice), Fancy Dan / Ricochet / Rand Robertson (voice), Fancy Dan / Homunculus #1 (voice)

Бен Діскін

Venom (voice), Eddie Brock / Venom (voice), Eddie Brock (voice)

Даран Норріс

J. Jonah Jameson (voice), J. Jonah Jameson / John Jameson (voice), J. Jonah Jameson / John Jameson / Colonel Jupiter (voice)

Кевін Майкл Річардсон

OsCorp Security Guard (voice), Big Man / Tombstone / L. Thompson Lincoln (voice), Coach Smith (voice), Principal Davis (voice), Tombstone / Principal Davis (voice), Tombstone (voice), Big Man / Coach Smith / Thug (voice)

Пітер МакНикол

Otto Octavius / Doctor Octopus (voice), Master Planner / Otto Octavius (voice), Doctor Octopus (voice), Master Planner (voice), Otto Octavius (voice), Master Planner / Doctor Octopus (voice)

Джон Дімаджіо

Hammerhead / Car Thief #2 (voice), Hammerhead (voice), Sandman (voice), Sandman / Hammerhead (voice), Flint Marko / Sandman / Hammerhead (voice), Flint Marko / Hammerhead (voice)

Ді Бредлі Бейкер

Curt Connors / Lizard (voice), Curt Connors (voice), Curt Connors / Homunculi (voice)

Крі Саммер

Glory Grant / Cobweb / Homunculus #2 (voice), Glory Grant (voice)

Crispin Freeman

Thug #1 (voice), Max Dillon / Electro (voice), Electro / Clerk (voice), Electro (voice)

Роберт Інґлунд

Vulture (voice), Adrian Toomes / Vulture (voice)

Dorian Harewood

Doc Bromwell (voice)

Тріша Гелфер

Felicia Hardy / Black Cat (voice), Black Cat (voice)

Miguel Ferrer

Silvermane (voice), Silvio Manfredi / Silvermane (voice)

Eric Vesbit

Sergei Kravinoff / Kraven the Hunter (voice), Kraven (voice)

Едвард Еснер

Ben Parker (voice)

Денні Трехо

Ox (voice)

Стен Лі

Stan (voice)

Стів Блум

Dilbert Trilby / Seymour O'Reily / Green Gobilin (voice)